標題: Sensitivity evaluation of rhodamine B hydrazide towards nitric oxide and its application for macrophage cells imaging
作者: Wu, Chi-Ming
Chen, Yen-Hao
Dayananda, Kasala
Shiue, Tsun-Wei
Hung, Chen-Hsiung
Liaw, Wen-Feng
Chen, Po-Yu
Wang, Yun-Ming
生物科技學系
Department of Biological Science and Technology
關鍵字: Nitric oxide;Fluorescent probe;Cell imaging;Rhodamine
公開日期: 5-Dec-2011
摘要: A colorless and non-fluorescent rhodamine derivative, rhodamine B hydrazide (RH), is applied to detect nitric oxide and form fluorescent rhodamine B (RB). The reaction mechanism of RH with NO is proposed in this study. The probe shows good stability over a broad pH range (pH > 4). Furthermore, fluorescence intensity of RH displays an excellent linearity to the NO concentration and the detection limit is as low as 20 nM. A 1000-fold fluorescence turn-on from a dark background was observed. Moreover, the selectivity study indicated that the fluorescence intensity increasing in the presence of NO was significantly higher than those of other reactive oxygen/nitrogen species. In exogenously generated NO detection study, clear intracellular red fluorescence was observed in the presence of S-nitroso-N-acetyl-D,L-penicillamine (SNAP, a kind of NO releasing agent). In endogenously generated NO detection study, increasing incubation time of RH with lipopolysaccharied (LPS) pre-treated cells could obtain a highly fluorescent cell image. These cell imaging results demonstrated that RH can efficiently penetrate into Raw 264.7 cells and be used for detection of exogenously and endogenously generated nitric oxide. (C) 2011 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.aca.2011.10.005
http://hdl.handle.net/11536/14901
ISSN: 0003-2670
DOI: 10.1016/j.aca.2011.10.005
期刊: ANALYTICA CHIMICA ACTA
Volume: 708
Issue: 1-2
起始頁: 141
結束頁: 148
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