標題: Functional transcranial photoacoustic micro-imaging of mouse cerebrovascular cross-section and hemoglobin oxygenation changes during forepaw electrical stimulation
作者: Liao, Lun-De
Chen, You-Yin
Lin, Chin-Teng
Chang, Jyh-Yeong
Li, Meng-Lin
電機工程學系
Department of Electrical and Computer Engineering
關鍵字: Functional imaging;photoacoustic microscopy;electrical stimulation;somatosensory cortex;vasodilatation;hemoglobin oxygenation
公開日期: 2011
摘要: In this study, we report on using a 50-MHz functional photoacoustic microscopy (PAM) to transcranially image the cross-section and hemoglobin oxygenation (SO(2)) changes of single mouse cortical vessels in response to left forepaw electrical stimulation. Three difference levels of the cortical vessels (i.e., with different-sized diameters of 350, 100 and 55 mu m) on activated regions were marked to measure their functional cross-section and SO(2) changes as a function of time. Electrical stimulation of the mouse left forelimb was applied to evoke functional changes in vascular dynamics of the mouse somatosensory cortex. The applied current pulses were with a pulse frequency of 3 Hz, pulse duration of 0.2 ms, and pulse amplitude of 2 mA. The cerebrovascular cross-section changes, which indicate changes in cerebral blood volume (CBV), were probed by images acquired at 570 nm, a hemoglobin isosbestic point, while SO(2) changes were monitored by the derivatives of 560-nm images normalized to 570-nm ones. The results show that vessel diameter and SO(2) were significantly dilated and increased when compared with those of the controlled ones. In summary, the PAM shows its promise as a new imaging modality for transcranially functional quantification of single vessel diameter (i.e., CBV) and SO(2) changes without any contrast agents applied during stimulation.
URI: http://hdl.handle.net/11536/14869
http://dx.doi.org/10.1117/12.874449
ISBN: 978-0-8194-8436-9
ISSN: 0277-786X
DOI: 10.1117/12.874449
期刊: PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2011
Volume: 7899
Appears in Collections:Conferences Paper


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