|標題:||Automated Dual-Chamber Sampling System to Follow Dynamics of Volatile Organic Compounds Emitted by Biological Specimens|
Urban, Pawel L.
Department of Applied Chemistry
|摘要:||Growth of microorganisms is often accompanied by the release of volatile organic compounds (VOCs). The released VOCs may qualitatively or quantitatively reflect the physiological states of microbial cultures. A number of VOCs are produced during microbial degradation of organic matter accompanying food spoilage. In order to characterize the dynamics of microbial VOC production, and enable time-dependent analysis, we have constructed a dual-chamber sampling system and coupled it online with mass spectrometry (MS). The biological specimen is placed in a gas-tight sampling chamber. A carrier gas is introduced to the chamber periodically to transfer the VOCs present in the specimen headspace to the atmospheric-pressure chemical-ionization interface of a triple-quadrupole mass spectrometer. A control or blank spectrum is recorded before the specimen spectrum is recorded at each time point, enabling signal comparison and subtraction. The custom-made electronic control unit, incorporating three Arduino microcontrollers, operates six pinch valves and a miniature air compressor and triggers MS-data acquisition. The automated dual-chamber sampling system was first tested using standard mixtures to verify its analytical performance. To demonstrate the usefulness of this system in the studies of microbial volatomes, we implemented it in real-time monitoring of the growth of bakers yeast (Saccharomyces cerevisiae), fructification of golden oyster mushroom (Pleurotus citrinopileatus), and microbial degradation of a food-related sample (Pacific white shrimp, Litopenaeus vannamei). The recorded VOC signals show characteristic temporal profiles with transient bands or plateaus corresponding to different stages of microbial growth and putrefaction processes.|
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