標題: In Vitro and in Vivo Imaging of Nitroxyl with Copper Fluorescent Probe in Living Cells and Zebrafish
作者: Palanisamy, Sathyadevi
Wang, Yu-Liang
Chen, Yu-Jen
Chen, Chiao-Yun
Tsai, Fu-Te
Liaw, Wen-Feng
Wang, Yun-Ming
分子醫學與生物工程研究所
Institute of Molecular Medicine and Bioengineering
關鍵字: nitroxyl;fluorescent probe;living system;zebrafish (Danio rerio)
公開日期: 1-Oct-2018
摘要: Nitroxyl (HNO) plays a critical role in many physiological processes which includes vasorelaxation in heart failure, neuroregulation, and myocardial contractility. Powerful imaging tools are required to obtain information for understanding the mechanisms involved in these in vivo processes. In order to develop a rapid and high sensitive probe for HNO detection in living cells and the zebrafish model organism, 2-((2-(benzothiazole-2yl)benzylidene) amino)benzoic acid (AbTCA) as a ligand, and its corresponding copper(II) complex Cu(II)-AbTCA were synthesized. The reaction results of Cu(II)-AbTCA with Angeli's salt showed that Cu(II)-AbTCA could detect HNO quantitatively in a range of 40-360 mu M with a detection limit of 9.05 mu M. Furthermore, Cu(II)-AbTCA is more selective towards HNO over other biological species including thiols, reactive nitrogen, and reactive oxygen species. Importantly, Cu(II)-AbTCA was successfully applied to detect HNO in living cells and zebrafish. The collective data reveals that Cu(II)-AbTCA could be used as a potential probe for HNO detection in living systems.
URI: http://dx.doi.org/10.3390/molecules23102551
http://hdl.handle.net/11536/148502
ISSN: 1420-3049
DOI: 10.3390/molecules23102551
期刊: MOLECULES
Volume: 23
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