標題: Dynamic photopatterning of cells in situ by Q-switched neodymium-doped yttrium ortho-vanadate laser
作者: Deka, Gitanjal
Okano, Kazunori
Kao, Fu-Jen
前瞻跨領域基礎科學中心
Center for Interdisciplinary Science
關鍵字: laser ablation;thermal transducer;cytophobic polymer;cell array;cellular migration
公開日期: 1-Jan-2014
摘要: Cellular micropattering has been increasingly adopted in quantitative biological experiments. A Q-switched pulsed neodymium-doped yttrium ortho-vanadate (Nd:YVO4) laser directed in-situ microfabrication technique for cell patterning is presented. A platform is designed uniquely to achieve laser ablation. The platform is comprised of thin gold coating over a glass surface that functions as a thermal transducer and is over-layered by a cell repellant polymer layer. Micropatterns are engraved on the platform, subsequently exposing specific cell adhesive micro-domains by ablating the gold-polymer coating photothermally. Experimental results indicate that the proposed approach is applicable under culture conditions, viable toward cells, and has a higher engraving speed. Possible uses in arraying isolated single cells on the platform are also shown. Additionally, based on those micro-patterns, dynamic cellular morphological changes and migrational speed in response to geometrical barriers are studied to demonstrate the potential applications of the proposed approach. Our results further demonstrate that cells in narrower geometry had elongated shapes and higher migrational speed than those in wider geometry. Importantly, the proposed approach will provide a valuable reference for efforts to study single cell dynamics and cellular migration related processes for areas such as cell division, wound healing, and cancer invasion. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
URI: http://dx.doi.org/10.1117/1.JBO.19.1.011012
http://hdl.handle.net/11536/147673
ISSN: 1083-3668
DOI: 10.1117/1.JBO.19.1.011012
期刊: JOURNAL OF BIOMEDICAL OPTICS
Volume: 19
Issue: 1
起始頁: 0
結束頁: 0
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