標題: Selective and Absolute Quantification of Endogenous Hypochlorous Acid with Quantum-Dot Conjugated Microbeads
作者: Yang, Yi-Cyun
Lu, Hsueh-Han
Wang, Wei-Ti
Liau, Ian
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 1-Nov-2011
摘要: Endogenous hypochlorous acid (Hod) secreted by leukocytes plays a critical role in both the immune defense of mammalians and the pathogenesis of-various diseases intimately related to inflammation. We report the first selective and absolute quantification of endogenous HOCl produced by leukocytes in vitro and in vivo with a novel quantum dot-based sensor. An activated human neutrophil secreted 6.5 +/- 0.9 x 10(8) HOCl molecules into its phagosome, and kinetic measurement for the secretions showed that the extracellular generation of HOCl was temporally retarded, but the quantity eventually attained a level comparable with its intraphagosomal counterpart with a delay of about 1.5 h. The quantity of HOCl secreted from the hepatic leukocytes of rats with or without stimulation of lipopolysaccharide was also determined. These results indicate a possibility to extend our approach to not only clinical settings for quantitative assessment of the bactericidal capability of isolated leukocytes of patients but also fundamental biomedical research that requires critical evaluation of the inflammatory response of animals.
URI: http://dx.doi.org/10.1021/ac202077x
http://hdl.handle.net/11536/14673
ISSN: 0003-2700
DOI: 10.1021/ac202077x
期刊: ANALYTICAL CHEMISTRY
Volume: 83
Issue: 21
起始頁: 8267
結束頁: 8272
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