標題: Diversity and enterotype in gut bacterial community of adults in Taiwan
作者: Liang, Chao
Tseng, Han-Chi
Chen, Hui-Mei
Wang, Wei-Chi
Chiu, Chih-Min
Chang, Jen-Yun
Lu, Kuan-Yi
Weng, Shun-Long
Chang, Tzu-Hao
Chang, Chao-Hsiang
Weng, Chen-Tsung
Wang, Hwei-Ming
Huang, Hsien-Da
生物科技學系
生物資訊及系統生物研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
關鍵字: Enterotype;16S rDNA;Next-generation sequencing;Gut microbiome;Predictive model
公開日期: 25-Jan-2017
摘要: Background: Gastrointestinal microbiota, particularly gut microbiota, is associated with human health. The biodiversity of gut microbiota is affected by ethnicities and environmental factors such as dietary habits or medicine intake, and three enterotypes of the human gut microbiome were announced in 2011. These enterotypes are not significantly correlated with gender, age, or body weight but are influenced by long-term dietary habits. However, to date, only two enterotypes (predominantly consisting of Bacteroides and Prevotella) have shown these characteristics in previous research; the third enterotype remains ambiguous. Understanding the enterotypes can improve the knowledge of the relationship between microbiota and human health. Results: We obtained 181 human fecal samples from adults in Taiwan. Microbiota compositions were analyzed using next-generation sequencing (NGS) technology, which is a culture-independent method of constructing microbial community profiles by sequencing 16S ribosomal DNA (rDNA). In these samples, 17,675,898 sequencing reads were sequenced, and on average, 215 operational taxonomic units (OTUs) were identified for each sample. In this study, the major bacteria in the enterotypes identified from the fecal samples were Bacteroides, Prevotella, and Enterobacteriaceae, and their correlation with dietary habits was confirmed. A microbial interaction network in the gut was observed on the basis of the amount of short-chain fatty acids, pH value of the intestine, and composition of the bacterial community (enterotypes). Finally, a decision tree was derived to provide a predictive model for the three enterotypes. The accuracies of this model in training and independent testing sets were 97.2 and 84.0%, respectively. Conclusions: We used NGS technology to characterize the microbiota and constructed a predictive model. The most significant finding was that Enterobacteriaceae, the predominant subtype, could be a new subtype of enterotypes in the Asian population.
URI: http://dx.doi.org/10.1186/s12864-016-3261-6
http://hdl.handle.net/11536/145996
ISSN: 1471-2164
DOI: 10.1186/s12864-016-3261-6
期刊: BMC GENOMICS
Volume: 18
起始頁: 0
結束頁: 0
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