標題: Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis
作者: Fang, Chih-Hsiang
Tsai, Pei-I
Huang, Shu-Wei
Sun, Jui-Sheng
Chang, Jenny Zwei-Chieng
Shen, Hsin-Hsin
Chen, San-Yuan
Lin, Feng Huei
Hsu, Lih-Tao
Chen, Yen-Chun
材料科學與工程學系
智慧型仿生系統研究中心
Department of Materials Science and Engineering
Biomimetic Systems Research Center
關鍵字: Peri-implant osteomyelitis;Magnetic nanoparticle;Hyperthermia;Biofilm
公開日期: 25-Jul-2017
摘要: Background: When bacteria colony persist within a biofilm, suitable drugs are not yet available for the eradication of biofilm-producing bacteria. The aim of this study is to study the effect of magnetic nano-particles-induced hyperthermia on destroying biofilm and promoting bactericidal effects of antibiotics in the treatment of osteomyelitis. Methods: Sixty 12-weeks-old male Wistar rats were used. A metallic 18G needle was implanted into the bone marrow cavity of distal femur after the injection of Methicillin-sensitive Staphylococcus aureus (MSSA). All animals were divided into 5 different treatment modalities. The microbiological evaluation, scanning electron microscope examination, radiographic examination and then micro-CT evaluation of peri-implant bone resorption were analyzed. Results: The pathomorphological characteristics of biofilm formation were completed after 40-days induction of osteomyelitis. The inserted implants can be heated upto 75 degrees C by magnetic heating without any significant thermal damage on the surrounding tissue. We also demonstrated that systemic administration of vancomycin [VC (i.m.)] could not eradicate the bacteria; but, local administration of vancomycin into the femoral canal and the presence of magnetic nanoparticles hyperthermia did enhance the eradication of bacteria in a biofilm-based colony. In these two groups, the percent bone volume (BV/TV: %) was significantly higher than that of the positive control. Conclusions: For the treatment of chronic osteomyelitis, we developed a new modality to improve antibiotic efficacy; the protection effect of biofilms on bacteria could be destroyed by magnetic nanoparticles-induced hyperthermia and therapeutic effect of systemic antibiotics could be enhanced.
URI: http://dx.doi.org/10.1186/s12879-017-2621-4
http://hdl.handle.net/11536/145836
ISSN: 1471-2334
DOI: 10.1186/s12879-017-2621-4
期刊: BMC INFECTIOUS DISEASES
Volume: 17
起始頁: 0
結束頁: 0
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