標題: Photothermal imaging of skeletal muscle mitochondria
作者: Tomimatsu, Toru
Miyazaki, Jun
Kano, Yutaka
Kobayashi, Takayoshi
電子物理學系
Department of Electrophysics
公開日期: 1-Jun-2017
摘要: The morphology and topology of mitochondria provide useful information about the physiological function of skeletal muscle. Previous studies of skeletal muscle mitochondria are based on observation with transmission, scanning electron microscopy or fluorescence microscopy. In contrast, photothermal (PT) microscopy has advantages over the above commonly used microscopic techniques because of no requirement for complex sample preparation by fixation or fluorescent-dye staining. Here, we employed the PT technique using a simple diode laser to visualize skeletal muscle mitochondria in unstained and stained tissues. The fine mitochondrial network structures in muscle fibers could be imaged with the PT imaging system, even in unstained tissues. PT imaging of tissues stained with toluidine blue revealed the structures of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria and the swelling behavior of mitochondria in damaged muscle fibers with sufficient image quality. PT image analyses based on fast Fourier transform (FFT) and Grey-level co-occurrence matrix (GLCM) were performed to derive the characteristic size of mitochondria and to discriminate the image patterns of normal and damaged fibers. (C) 2017 Optical Society of America
URI: http://dx.doi.org/10.1364/BOE.8.002965
http://hdl.handle.net/11536/145746
ISSN: 2156-7085
DOI: 10.1364/BOE.8.002965
期刊: BIOMEDICAL OPTICS EXPRESS
Volume: 8
Issue: 6
起始頁: 2965
結束頁: 2975
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