標題: Studying anti-oxidative properties of inclusion complexes of alpha-lipoic acid with gamma-cyclodextrin in single living fission yeast by confocal Raman microspectroscopy
作者: Noothalapati, Hemanth
Ikarashi, Ryo
Iwasaki, Keita
Nishida, Tatsuro
Kaino, Tomohiro
Yoshikiyo, Keisuke
Terao, Keiji
Nakata, Daisuke
Ikuta, Naoko
Ando, Masahiro
Hamaguchi, Hiro-o
Kawamukai, Makoto
Yamamoto, Tatsuyuki
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Raman microspectroscopy;Fission yeast;Lipoic acid;Cyclodextrin;Antioxidant
公開日期: 15-May-2018
摘要: alpha-lipoic acid (ALA) is an essential cofactor for many enzyme complexes in aerobic metabolism, especially in mitochondria of eukaryotic cells where respiration takes place. It also has excellent anti-oxidative properties. The acid has two stereo-isomers, R- and S-lipoic acid (R-LA and S-LA), but only the R-LA has biological significance and is exclusively produced in our body. A mutant strain of fission yeast, Delta dpsl, cannot synthesize coenzyme Q10, which is essential during yeast respiration, leading to oxidative stress. Therefore, it shows growth delay in the minimal medium. We studied anti-oxidant properties of ALA in its free form and their inclusion complexes with gamma-cyclodextrin using this mutant yeast model. Both free forms R- and S-LA as well as 1:1 inclusion complexes with gamma-cyclodexhin recovered growth of Delta dps1 depending on the concentration and form. However, it has no effect on the growth of wild type fission yeast strain at all. Raman microspectroscopy was employed to understand the anti-oxidant property at the molecular level. A sensitive Raman band at 1602 cm(-1) was monitored with and without addition of ALAs. It was found that 0.5 mM and 1.0 mM concentrations of ALAs had similar effect in both free and inclusion forms. At 2.5 mM ALAs, free forms inhibited the growth while inclusion complexes helped in recovered. 5.0 mM ALA showed inhibitory effect irrespective of form. Our results suggest that the Raman band at 1602 cm(-1) is a good measure of oxidative stress in fission yeast. (C) 2018 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.saa.2018.02.011
http://hdl.handle.net/11536/144917
ISSN: 1386-1425
DOI: 10.1016/j.saa.2018.02.011
期刊: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume: 197
起始頁: 237
結束頁: 243
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