標題: Lipopolysaccharide-Induced Nitric Oxide, Prostaglandin E2, and Cytokine Production of Mouse and Human Macrophages Are Suppressed by Pheophytin-b
作者: Lin, Chun-Yu
Wang, Wen-Hung
Chen, Shin-Huei
Chang, Yu-Wei
Hung, Ling-Chien
Chen, Chung-Yi
Chen, Yen-Hsu
生醫工程研究所
Institute of Biomedical Engineering
關鍵字: pheophytin-b;nitric oxide;prostaglandin E2;cytokine;macrophages;lipopolysaccharide
公開日期: 1-Dec-2017
摘要: Sepsis is an overwhelming systemic response to infection that frequently results in tissue damage, organ failure, and even death. Nitric oxide (NO), prostaglandin E2 (PGE2), and cytokine overproduction are thought to be associated with the immunostimulatory cascade in sepsis. In the present study, we analyzed the anti-inflammatory efficacy of the pheophytin-b on both RAW264.7 murine macrophage and purified human CD14(+) monocytes stimulated with lipopolysaccharide (LPS) and elucidated the mechanisms by analyzing the cell signaling pathways known to be activated in sepsis. Pheophytin-b suppressed the overexpression of NO, PGE2, and cytokines in LPS-stimulated macrophages without inducing cytotoxicity. It also reduced NOS2 and COX-2 mRNA and protein levels. The inhibitory effects on NO, PGE2, and cytokine overproduction arose from the suppression of STAT-1 and PI3K/Akt pathways; no changes in NF-kappa B, MAPK, and AP-1 signaling were detected. Thus, pheophytin-b may represent a potential candidate to beneficially modulate the inflammatory response in sepsis.
URI: http://dx.doi.org/10.3390/ijms18122637
http://hdl.handle.net/11536/144308
ISSN: 1422-0067
DOI: 10.3390/ijms18122637
期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume: 18
Issue: 12
起始頁: 0
結束頁: 0
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