標題: Functional Characterization of Acinetobacter baumannii Lacking the RNA Chaperone Hfq
作者: Kuo, Han-Yueh
Chao, Hsuan-Hao
Liao, Po-Cheng
Hsu, Long
Chang, Kai-Chih
Tung, Chi-Hua
Chen, Chang-Hua
Liou, Ming-Li
電子物理學系
Department of Electrophysics
關鍵字: Acinetobacter baumannii;Hfq;adhesion;invasion;outer membrane vesicles
公開日期: 27-Oct-2017
摘要: The RNA chaperone Hfq is involved in the riboregulation of diverse genes via small RNAs. Recent studies have demonstrated that Hfq contributes to the stress response and the virulence of several pathogens, and the roles of Hfq vary among bacterial species. Here, we attempted to elucidate the role of Hfq in Acinetobacter baumannii ATCC 17978. In the absence of hfq, A. baumannii exhibited retarded cell growth and was highly sensitive to environmental stress, including osmotic and oxidative pressure, pH, and temperature. Compared to the wild-type, the Hfq mutant had reduced outer membrane vesicles secretion and fimbriae production as visualized by atomic force microscopy. The absence of hfq reduced biofilm formation, airway epithelial cell adhesion and invasion, and survival in macrophage. Further, the hfq mutant induced significantly higher IL-8 levels in airway epithelial cells, which would promote bacterial clearance by the host. In addition to results similar to those reported for other bacteria, our findings demonstrate that Hfq is required in the regulation of the iron-acquisition system via downregulating the bauA and basD genes, the stress-related outer membrane proteins carO, A1S_0820, ompA, and nlpE, and the stress-related cytosolic proteins uspA and groEL. Our data indicate that Hfq plays a critical role in environmental adaptation and virulence in A. baumannii by modulating stress responses, surface architectures, and virulence factors. This study is the first to illustrate the functional role of Hfq in A. baumannii.
URI: http://dx.doi.org/10.3389/fmicb.2017.02068
http://hdl.handle.net/11536/143995
ISSN: 1664-302X
DOI: 10.3389/fmicb.2017.02068
期刊: FRONTIERS IN MICROBIOLOGY
Volume: 8
起始頁: 0
結束頁: 0
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