標題: Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold
作者: Yang, Tien-Chun
Chuang, Jen-Hua
Buddhakosai, Waradee
Wu, Wen-Ju
Lee, Chen-Ju
Chen, Wun-Syuan
Yang, Yi-Ping
Li, Ming-Chia
Peng, Chi-Hsien
Chen, Shih-Jen
生物科技學系
Department of Biological Science and Technology
關鍵字: nano-imprinted;scaffold;RGC;axon outgrowth;elongation;orientation
公開日期: 1-Sep-2017
摘要: Optic neuropathies, such as glaucoma and Leber's hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the correct location of the retina. The use of appropriate scaffold can promote the proper axon growth. Recently, biocompatible materials have been integrated into the medical field, such as tissue engineering and reconstruction of damaged tissues or organs. We, herein, utilized nano-imprinting to create a scaffold mimicking the in vitro tissue microarchitecture, and guiding the axonal growth and orientation of the RGCs. We observed that the robust, long, and organized axons of human induced pluripotent stem cell (iPSC)-derived RGCs projected axially along the scaffold grooves. The RGCs grown on the scaffold expressed the specific neuronal biomarkers indicating their proper functionality. Thus, based on our in vitro culture system, this device can be useful for the neurophysiological analysis and transplantation for ophthalmic neuropathy treatment.
URI: http://dx.doi.org/10.3390/ijms18092013
http://hdl.handle.net/11536/143847
ISSN: 1422-0067
DOI: 10.3390/ijms18092013
期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume: 18
Issue: 9
起始頁: 0
結束頁: 0
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