標題: MultiProtident: Identifying proteins using database search and protein-protein interactions
作者: Huang, HD
Lee, TY
Wu, LC
Lin, FM
Juan, HF
Horng, JT
Tsou, AP
生物科技學系
生物資訊及系統生物研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
關鍵字: protein identification;peptide mass fingerprint;protein-protein interaction
公開日期: 1-May-2005
摘要: Protein identification is important in proteomics. Proteomic analyses based on mass spectra (MS) constitute innovative ways to identify the components of protein complexes. Instruments can obtain the mass spectrum to an accuracy of 0.01 Da or better, but identification errors are inevitable. This study shows a novel tool, MultiProtldent, which can identify proteins using additional information about protein-protein interactions and protein functional associations. Both single and multiple Peptide Mass Fingerprints (PMFs) are input to MultiProtldent, which matches the PMFs to a theoretical peptide mass database. The relationships or interactions among proteins are considered to reduce false positives in PMF matching. Experiments to identify protein complexes reveal that MultiProtldent is highly promising. The website associated with this study is http://dbms104.csie.ncu.edu.tw/.
URI: http://dx.doi.org/10.1021/pr0498335
http://hdl.handle.net/11536/13757
ISSN: 1535-3893
DOI: 10.1021/pr0498335
期刊: JOURNAL OF PROTEOME RESEARCH
Volume: 4
Issue: 3
起始頁: 690
結束頁: 697
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