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dc.contributor.authorSar, Jenny I-Chunen_US
dc.contributor.authorLin, Yen-Zhuen_US
dc.contributor.authorLin, Chung-Kweien_US
dc.contributor.authorLiu, Tse-Yingen_US
dc.contributor.authorChen, San-Yuanen_US
dc.date.accessioned2017-04-21T06:49:27Z-
dc.date.available2017-04-21T06:49:27Z-
dc.date.issued2016-10-15en_US
dc.identifier.issn0257-8972en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.surfcoat.2016.03.088en_US
dc.identifier.urihttp://hdl.handle.net/11536/136245-
dc.description.abstractIn this study, the dicalcium phosphate dehydrate (DCPD)/carboxymethyl hexanoyl chitosan (CHC) hybrid coating layer with sequential release of BMP-2 and TGF-beta 1 was deposited onto Ti6Al4V substrate by electrochemical co-precipitation method. Different concentrations of amphiphatic chitosan derivative (carboxymethyl hexanoyl chitosan, CHC), BMP-2 and TGF-beta 1-loaded PLA microspheres (PLA/TGF-beta 1 MSs) were co-precipitated with the precursor of DCPD. The resulting DCPD/CHC hybrid coating incorporated with BMP-2 and PLA/TGF-beta 1 MSs was designed for improving the osteoconductive and osteoinductive functionalities of Ti6Al4V. FTIR spectroscopy and X-ray diffraction were used to characterize these hybrid coatings. The drug release profile, MC3T3-E1 cell proliferation and alkaline phosphatase activity were used to investigate the functionality of these hybrid coatings. It was found that the incorporation of CHC could be used as a mean to manipulate the release behavior of growth factor from the DCPD/CHC hybrid coating. In addition, the BMP-2- and PLA/TGF-beta 1 MSs-incorporated DCPD/CHC hybrid coating exhibited a sequential release of BMP-2 and TGF-beta 1 during the first and second two weeks, respectively. Importantly, the DCPD/CHC hybrid coating immersed in simulated body fluid gradually transformed to hydroxyapatite phase. Hence, the BMP-2 and PLA/TGF-beta 1 MSs-incorporated DCPD/CHC hybrid coating exhibited the best cell viability and the highest ALP activity after 14 days. The biocompatible hybrid coating with sequential release functionality proposed in the present study can enhance the osseointegration process and possess promising potential for clinical applications in bone implants and tissue engineering. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectDCPDen_US
dc.subjectCHCen_US
dc.subjectElectrodepositionen_US
dc.subjectBMP-2en_US
dc.subjectTGF-beta 1en_US
dc.titleGrowth factors-loaded calcium phosphate/polymer hybrid coating with sequential release behavior prepared via electrochemical deposition methoden_US
dc.typeArticle; Proceedings Paperen_US
dc.identifier.doi10.1016/j.surfcoat.2016.03.088en_US
dc.identifier.journalSURFACE & COATINGS TECHNOLOGYen_US
dc.citation.volume303en_US
dc.citation.spage237en_US
dc.citation.epage243en_US
dc.contributor.department材料科學與工程學系zh_TW
dc.contributor.department生物科技學院zh_TW
dc.contributor.departmentDepartment of Materials Science and Engineeringen_US
dc.contributor.departmentCollege of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000384857300035en_US
dc.citation.woscount0en_US
Appears in Collections:Conferences Paper