標題: Computer method for ELISA spot assay analysis
作者: Lin, CY
Ching, YT
Wu-Hsieh, BA
資訊工程學系
Department of Computer Science
關鍵字: ELISA;spot;matched filter;segmentation;color analysis;CIE
公開日期: 1-八月-2005
摘要: The ELISA (enzyme-linked immunosorbent assay) spot assay is a method widely used by immunologists to enumerate cytokine-producing cells within a specific cell population. The ELISA results are presented in an image containing numerous colored spots. We present a method to identify the spots in the image and report on important statistics regarding them. The proposed method employs color analysis in the CIE Luv color space and matched filter technique. The system is trained to obtain a standard color for the spots and calculate the color differences between the spots and background in the Luv space. Matched filters are then used to remove noise and enhance the spots in the color difference map. Intensity thresholding is applied to obtain a binary image in which the pixels in the spots have a grayscale of 1 while the grayscale of the other pixels is 0. A software system is implemented, based on this method, to help immunologists analyze the results obtained from the. ELISA. (c) 2005 Society of Photo-Optical Instrumentation Engineers.
URI: http://dx.doi.org/10.1117/1.2009767
http://hdl.handle.net/11536/13462
ISSN: 0091-3286
DOI: 10.1117/1.2009767
期刊: OPTICAL ENGINEERING
Volume: 44
Issue: 8
結束頁: 
顯示於類別:期刊論文


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  1. 000232596000010.pdf