Title: Functional Supramolecular Polymers: A Fluorescent Microfibrous Network in a Supramolecular Hydrogel for High-Contrast Live Cell-Material Imaging in 3D Environments
Authors: Hsu, Shu-Min
Wu, Fang-Yi
Cheng, Hsun
Huang, Yu-Tang
Hsieh, Yi-Ru
Tseng, Dion Tzu-Huan
Yeh, Mei-Yu
Hung, Shih-Chieh
Lin, Hsin-Chieh
Department of Materials Science and Engineering
Issue Date: 21-Sep-2016
Abstract: A new bottom-up strategy based on aromatic peptide amphiphile is developed for a high-contrast visualization of 3D live cell-material imaging-something that has been difficult to achieve previously because of the problems associated with the diffraction of light by the nanosized peptide materials and the aggregation-caused quenching of aggregated pi-conjugated fluorophores in the nanostructures. This study reports an example of a novel supramolecular hydrogelator, naphthaleneimide-phenylalanine (NI-Phe), which forms a self-supporting hydrogel displaying a unique microfibrous network and promising aggregation-induced emission characteristics at pH 7.4. The storage modulus of the NI-Phe gel supports the mass of a cell for 3D cell culturing. This work illustrates a new dopant-free supramolecular approach, complementary to well-established doping procedures that should facilitate the development of live cell imaging in 3D scaffolding materials.
URI: http://dx.doi.org/10.1002/adhm.201600342
ISSN: 2192-2640
DOI: 10.1002/adhm.201600342
Volume: 5
Issue: 18
Begin Page: 2406
End Page: 2412
Appears in Collections:Articles