標題: Fluorescent tracer of dopamine enables selective labelling and interrogation of dopaminergic amacrine cells in the retina of living zebrafish
作者: Lin, Hui-Jen
Hong, Zhen-Yi
Li, Yaw-Kuen
Liau, Ian
生物科技學系
應用化學系
Department of Biological Science and Technology
Department of Applied Chemistry
公開日期: 1-Jan-2016
摘要: The retinal dopaminergic amacrine cell plays multiple roles in vision; it optimizes the encoding of visual stimuli, serves as an output of the circadian rhythm, and influences trophic processes. Specific identification of such cells in vivo remains challenging. A fluorescent tracer that mimics natural dopamine would help elucidate the distribution and the functional roles of dopaminergic neurons. Here we report a fluorescent tracer of dopamine for the selective labeling and study of retinal dopaminergic amacrine cells in vivo. This tracer has low toxicity and effective selectivity, and enables fluorescent determination of the evoked release of dopamine and the femtosecond laser ablation of single dopaminergic amacrine cells in living zebrafish. Our approach is readily adaptable by other research groups, and is expected to accelerate biomedical research in domains related to the pathophysiology of retinal dopaminergic amacrine cells.
URI: http://dx.doi.org/10.1039/c6ra13073a
http://hdl.handle.net/11536/134171
ISSN: 2046-2069
DOI: 10.1039/c6ra13073a
期刊: RSC ADVANCES
Volume: 6
Issue: 75
起始頁: 71589
結束頁: 71595
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