Title: Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy
Authors: Miyazaki, Jun
Iida, Tadatsune
Tanaka, Shinji
Hayashi-Takagi, Akiko
Kasai, Haruo
Okabe, Shigeo
Kobayashi, Takayoshi
Department of Electrophysics
Issue Date: 1-May-2016
Abstract: A fast, high-sensitivity photothermal microscope was developed by implementing a spatially segmented balanced detection scheme into a laser scanning microscope. We confirmed a 4.9 times improvement in signal-to-noise ratio in the spatially segmented balanced detection compared with that of conventional detection. The system demonstrated simultaneous bi-modal photothermal and confocal fluorescence imaging of transgenic mouse brain tissue with a pixel dwell time of 20 mu s. The fluorescence image visualized neurons expressing yellow fluorescence proteins, while the photothermal signal detected endogenous chromophores in the mouse brain, allowing 3D visualization of the distribution of various features such as blood cells and fine structures probably due to lipids. This imaging modality was constructed using compact and cost-effective laser diodes, and will thus be widely useful in the life and medical sciences. (C) 2016 Optical Society of America
URI: http://dx.doi.org/10.1364/BOE.7.001702
ISSN: 2156-7085
DOI: 10.1364/BOE.7.001702
Volume: 7
Issue: 5
Begin Page: 1702
End Page: 1710
Appears in Collections:Articles