標題: Label-free Chemical Imaging of Fungal Spore Walls by Raman Microscopy and Multivariate Curve Resolution Analysis
作者: Noothalapati, Hemanth
Sasaki, Takahiro
Kaino, Tomohiro
Kawamukai, Makoto
Ando, Masahiro
Hamaguchi, Hiro-o
Yamamoto, Tatsuyuki
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 9-Jun-2016
摘要: Fungal cell walls are medically important since they represent a drug target site for antifungal medication. So far there is no method to directly visualize structurally similar cell wall components such as alpha-glucan, beta-glucan and mannan with high specificity, especially in a label-free manner. In this study, we have developed a Raman spectroscopy based molecular imaging method and combined multivariate curve resolution analysis to enable detection and visualization of multiple polysaccharide components simultaneously at the single cell level. Our results show that vegetative cell and ascus walls are made up of both alpha- and beta-glucans while spore wall is exclusively made of alpha-glucan. Co-localization studies reveal the absence of mannans in ascus wall but are distributed primarily in spores. Such detailed picture is believed to further enhance our understanding of the dynamic spore wall architecture, eventually leading to advancements in drug discovery and development in the near future.
URI: http://dx.doi.org/10.1038/srep27789
http://hdl.handle.net/11536/133733
ISSN: 2045-2322
DOI: 10.1038/srep27789
期刊: SCIENTIFIC REPORTS
Volume: 6
起始頁: 0
結束頁: 0
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