標題: Ran-dependent TPX2 activation promotes acentrosomal microtubule nucleation in neurons
作者: Chen, Wen-Shin
Chen, Yi-Ju
Huang, Yung-An
Hsieh, Bing-Yuan
Chiu, Ho-Chieh
Kao, Pei-Ying
Chao, Chih-Yuan
Hwang, Eric
生物科技學系
生物資訊及系統生物研究所
分子醫學與生物工程研究所
生物資訊研究中心
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
Institute of Molecular Medicine and Bioengineering
Center for Bioinformatics Research
公開日期: 13-Feb-2017
摘要: The microtubule (MT) cytoskeleton is essential for the formation of morphologically appropriate neurons. The existence of the acentrosomal MT organizing center in neurons has been proposed but its identity remained elusive. Here we provide evidence showing that TPX2 is an important component of this acentrosomal MT organizing center. First, neurite elongation is compromised in TPX2-depleted neurons. In addition, TPX2 localizes to the centrosome and along the neurite shaft bound to MTs. Depleting TPX2 decreases MT formation frequency specifically at the tip and the base of the neurite, and these correlate precisely with the regions where active GTP-bound Ran proteins are enriched. Furthermore, overexpressing the downstream effector of Ran, importin, compromises MT formation and neuronal morphogenesis. Finally, applying a Ran-importin signaling interfering compound phenocopies the effect of TPX2 depletion on MT dynamics. Together, these data suggest a model in which Ran-dependent TPX2 activation promotes acentrosomal MT nucleation in neurons.
URI: http://dx.doi.org/10.1038/srep42297
http://hdl.handle.net/11536/133161
ISSN: 2045-2322
DOI: 10.1038/srep42297
期刊: SCIENTIFIC REPORTS
Volume: 7
起始頁: 0
結束頁: 0
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