Title: Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis
Authors: Lin, Yan-Ren
Li, Chao-Jui
Syu, Shih-Han
Wen, Cheng-Hao
Buddhakosai, Waradee
Wu, Han-Ping
Chen, Cheng Hsu
Lu, Huai-En
Chen, Wen-Liang
Department of Biological Science and Technology
Issue Date: 2016
Abstract: Postcardiac arrest acidosis can decrease survival. Effective medications without adverse side effects are still not well characterized. We aimed to analyze whether early administration of glutamine could improve survival and protect cardiomyocytes from postcardiac arrest acidosis using animal and cell models. Forty Wistar rats with postcardiac arrest acidosis (blood pH < 7.2) were included. They were divided into study (500 mg/kg L-alanyl-L-glutamine, n = 20) and control (normal saline,n = 20) groups. Each of the rats received resuscitation. The outcomes were compared between the two groups. In addition, cardiomyocytes derived from human induced pluripotent stem cells were exposed to HBSS with different pH levels (7.3 or 6.5) or to culture medium (control). Apoptosis-related markers and beating function were analyzed. We found that the duration of survival was significantly longer in the study group (n < 0.05). In addition, in pH 6.5 or pH 7.3 HBSS buffer, the expression levels of cell stress (p53) and apoptosis (caspase-3, Bcl-xL) markers were significantly lower in cardiomyocytes treated with 50 mM L-glutamine than those without L-glutamine (RT-PCR). L-glutamine also increased the beating function of cardiomyocytes, especially at the lower pH level (6.5). More importantly, glutamine decreased cardiomyocyte apoptosis and increased these cells\' beating function at a low pH level.
URI: http://dx.doi.org/10.1155/2016/2106342
ISSN: 2314-6133
DOI: 10.1155/2016/2106342
Appears in Collections:Articles