標題: beta-Lactoglobulin Influences Human Immunity and Promotes Cell Proliferation
作者: Tai, Chun San
Chen, Yi Yun
Chen, Wen Liang
生物科技學系
分子醫學與生物工程研究所
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
公開日期: 2016
摘要: beta-Lactoglobulin (LG) is suspected to enhance ormodulate humanimmune responses. Moreover, LGis also hypothesized to increase human cell proliferation. However, these potential functions of LG have not been directly or thoroughly addressed. In this study, we demonstrated that LG is a potent stimulator of cell proliferation using a hybridoma cell (a splenocyte fused with amyeloma cell) model. LG\'s ability to promote cell proliferation was lost when the protein is denatured. To further investigate the influence of LG\'s conformation on cell proliferation, we chemically modified LG by either carboxymethylation (CM) or acetylation and observed significantly reduced cell proliferation when the protein structure was altered. Furthermore, we proved that LG enhances cell proliferation via receptor-mediated membrane IgM receptor. These data indicated that nondenatured LG is the major component in milk that modulates cell proliferation. Collectively, our study showed that LG plays a key role in enhancing immune responses by promoting cell proliferation through IgM receptor.
URI: http://dx.doi.org/10.1155/2016/7123587
http://hdl.handle.net/11536/132912
ISSN: 2314-6133
DOI: 10.1155/2016/7123587
期刊: BIOMED RESEARCH INTERNATIONAL
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