標題: LWD-TCP complex activates the morning gene CCA1 in Arabidopsis
作者: Wu, Jing-Fen
Tsai, Huang-Lung
Joanito, Ignasius
Wu, Yi-Chen
Chang, Chin-Wen
Li, Yi-Hang
Wang, Ying
Hong, Jong Chan
Chu, Jhih-Wei
Hsu, Chao-Ping
Wu, Shu-Hsing
生物科技學系
生物資訊及系統生物研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
公開日期: 13-Oct-2016
摘要: A double-negative feedback loop formed by the morning genes CIRCADIAN CLOCK ASSOCIATED1 (CCA1)/LATE ELONGATED HYPOCOTYL (LHY) and the evening gene TIMING OF CAB EXPRESSION1 (TOC1) contributes to regulation of the circadian clock in Arabidopsis. A 24-h circadian cycle starts with the peak expression of CCA1 at dawn. Although CCA1 is targeted by multiple transcriptional repressors, including PSEUDO-RESPONSE REGULATOR9 (PRR9), PRR7, PRR5 and CCA1 HIKING EXPEDITION (CHE), activators of CCA1 remain elusive. Here we use mathematical modelling to infer a co-activator role for LIGHT-REGULATED WD1 (LWD1) in CCA1 expression. We show that the TEOSINTE BRANCHED 1-CYCLOIDEA-PCF20 (TCP20) and TCP22 proteins act as LWD-interacting transcriptional activators. The concomitant binding of LWD1 and TCP20/TCP22 to the TCP-binding site in the CCA1 promoter activates CCA1. Our study reveals activators of the morning gene CCA1 and provides an action mechanism that ensures elevated expression of CCA1 at dawn to sustain a robust clock.
URI: http://dx.doi.org/10.1038/ncomms13181
http://hdl.handle.net/11536/132634
ISSN: 2041-1723
DOI: 10.1038/ncomms13181
期刊: NATURE COMMUNICATIONS
Volume: 7
起始頁: 0
結束頁: 0
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