標題: Plant Cell Wall-Penetrable, Redox-Responsive Silica Nanoprobe for the Imaging of Starvation-Induced Vesicle Trafficking
作者: Huang, Xin-Chun
Inoue-Aono, Yuko
Moriyasu, Yuji
Hsieh, Pei-Ying
Tu, Wei-Ming
Hsiao, Shae-Chien
Jane, Wan-Neng
Hsu, Hsin-Yun
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 18-Oct-2016
摘要: Autophagy is a self-protection process against reactive oxygen species (ROS). The intracellular level of ROS increased when cells were cultured under nutrient starvation. Antioxidants such as glutathione and ascorbic acid play an important role in ROS removal. However, the cellular redox state In the autophagic pathway is still unclear. Herein, we developed a new redox-sensitive probe with a disulfide-linked silica scaffold to enable the sensing of the reduction environment in cell organelles. This redox-responsive silica nanoprobe (ReSiN) could penetrate the plant cell wall and release fluorescent molecules in; response to redox states. By applying the ReSiN to tobacco BY-2 cells and tracing the distribution of fluorescence, we found a higher reducing potential in. the central vacuole than in the autolysosomes. Upon cysteine protease inhibitor (E64-c) treatment in sucrose free medium, the disulfide-silica structures. of the ReSiNs were broken down in the vacuoles but Were not degraded and were accumulated in the autolysosomes. These results reveal the feasibility of our nanoprobe for monitoring the endocytic and Macroautophagic pathways. These pathways merge upstream of the-central vacuole, which is the final destination of both pathways. In addition, different redox potentials were observed in the autophagic pathway. Finally, the expression of the autophagy-related protein (Atg8) fused with green fluorescence protein confirmed that the ReSiN treatment itself did not induce the autophagic pathway under normal physiological conditions, indicating the versatility of this nanoprobe in studying stimuli triggered autophagy-related trafficking.
URI: http://dx.doi.org/10.1021/acs.analchem.6b02920
http://hdl.handle.net/11536/132628
ISSN: 0003-2700
DOI: 10.1021/acs.analchem.6b02920
期刊: ANALYTICAL CHEMISTRY
Volume: 88
Issue: 20
起始頁: 10231
結束頁: 10236
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