標題: Inhibition of breast cancer with transdermal tamoxifen-encapsulated lipoplex
作者: Lin, Yu-Ling
Chen, Chia-Hung
Wu, Hsin-Yi
Tsai, Nu-Man
Jian, Ting-Yan
Chang, Yuan-Ching
Lin, Chi-Hsin
Wu, Chih-Hsiung
Hsu, Fei-Ting
Leung, Ting Kai
Liao, Kuang-Wen
生物科技學系
分子醫學與生物工程研究所
生物資訊研究中心
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
Center for Bioinformatics Research
關鍵字: Lipo-PEG-PEI complex;Tamoxifen;Transdermal treatment;Breast cancer
公開日期: 19-Feb-2016
摘要: Background: Tamoxifen is currently used for the treatment of both early and advanced estrogen receptor (ER) positive breast cancer in pre- and post-menopausal women. However, using tamoxifen routinely to inhibit endogenous or exogenous estrogen effects is occasionally difficult because of its potential side effects. Objectives: The aim of this study is to design a local drug delivery system to encapsulate tamoxifen for observing their efficacy of skin penetration, drug accumulation and cancer therapy. Methods: A cationic liposome-PEG-PEI complex (LPPC) was used as a carrier for the encapsulation of tamoxifen and forming 'LPPC/TAM' for transdermal release. The cytotoxicity of LPPC/TAM was analyzed by MTT. The skin penetration, tumor growth inhibition and organ damages were measured in xenograft mice following transdermal treatment. Results: LPPC/TAM had an average size less than 270 nm and a zeta-potential of approximately 40 mV. LPPC/TAM displayed dramatically increased the cytotoxic activity in all breast cancer cells, especially in ER-positive breast cancer cells. In vivo, LPPC drug delivery helped the fluorescent dye penetrating across the skim and accumulating rapidly in tumor area. Administration of LPPC/TAM by transdermal route inhibited about 86 % of tumor growth in mice bearing BT474 tumors. This local treatment of LPPC/TAM did not injury skin and any organs. Conclusion: LPPC-delivery system provided a better skin penetration and drug accumulation and therapeutic efficacy. Therefore, LPPC/TAM drug delivery maybe a useful transdermal tool of drugs utilization for breast cancer therapy.
URI: http://dx.doi.org/10.1186/s12951-016-0163-3
http://hdl.handle.net/11536/132608
ISSN: 1477-3155
DOI: 10.1186/s12951-016-0163-3
期刊: JOURNAL OF NANOBIOTECHNOLOGY
Volume: 14
起始頁: 0
結束頁: 0
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