標題: Fluorescent nanodiamonds for specifically targeted bioimaging: Application to the interaction of transferrin with transferrin receptor
作者: Weng, Mao-Feng
Chiang, Su-Yu
Wang, Niann-Shiah
Niu, Huan
應用化學系
Department of Applied Chemistry
關鍵字: Nanodiamonds;Transferrin;Confocal fluorescence images;Fluorescence lifetime
公開日期: 1-Feb-2009
摘要: Fluorescent nanodiamonds (FND)as specifically targeted probes to image the interactions of transferrins (TF) with their receptors that were over-expressed on HeLa cells were studied in this work. The 100-nm diamonds were irradiated with a 2.5-MeV ion beam and thermally annealed to emit bright fluorescence in the region 550-800 nm upon excitation at 514.5 nm; accordingly, the interference of cellular autofluorescence at 520-650 nm was eliminated significantly on detecting fluorescence at >660 nm. FND-Tf bioconjugates were produced via the formation of an amide bond between carboxylated FND and transferrin and used to target transferrin receptors (TfR) on HeLa cells for activity testing. The receptor-mediated uptake of FND-Tf bioconjugates into HeLa cells was confirmed from confocal fluorescence images; in contrast, the uptake of FND-Tf bioconjugates on presaturated receptors or carboxylated FND on HeLa cells was unobserved. We measured also the fluorescence spectra and lifetimes of carboxylated FND, FND-Tf bioconjugates and FND-Tf-TfR complexes for characterization. Their measured lifetimes are 11.8 +/- 0.1, 11.6 +/- 0.1 and 12.+/- 0.1 ns, respectively. and fluorescence spectra are comparable except that the two zero-phonon lines of FND-Tf-TfR complexes become indistinct. Both results show that the surface effects of chemical interactions on the emission center of FND are negligible. (C) 2008 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.diamond.2008.07.012
http://hdl.handle.net/11536/12845
ISSN: 0925-9635
DOI: 10.1016/j.diamond.2008.07.012
期刊: DIAMOND AND RELATED MATERIALS
Volume: 18
Issue: 2-3
起始頁: 587
結束頁: 591
Appears in Collections:Conferences Paper


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