標題: Matrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cycles
作者: Yang, Wen-Jui
Liu, Fon-Chang
Hsieh, Jih-Sheng
Chen, Ching-Hung
Hsiao, Shun-Yu
Lin, Chih-Sheng
生物科技學系
Department of Biological Science and Technology
關鍵字: Follicular fluid;in vitro fertilization;Matrix metalloproteinase;Oocytes maturation;Tissue inhibitor of matrix metalloproteinase
公開日期: 4-Sep-2015
摘要: Background: To determine whether matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMP-1 and TIMP-2) in human follicular fluid, have any relationships with oocyte maturation in vivo and subsequent fertilization during in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycles. Methods: The follicular fluids were obtained from 150 female patients undergoing IVF/ICSI cycles and a total of 1504 oocytes were retrieved for analysis. MMP-2 and MMP-9 activities were measured using zymography assay. TIMP-1 and TIMP-2 concentrations were quantitatively assessed using enzyme-linked immunosorbent assay (ELISA). Results: Human follicular fluid MMP-2 level was significantly associated with the rate of maturity of oocytes (P < 0.001). Furthermore, the MMP-2 was significantly associated with the higher fertilization rate (P < 0.01). There was no significant correlation between follicular MMP-9 and the maturation rate of oocytes. The TIMP-1 and TIMP-2 also showed no correlation with the oocyte maturation rate. Conclusions: The level of gelatinase MMP-2 in human follicular fluid might be a reliable marker of mature oocytes during IVF/ICSI cycles. Furthermore, the MMP-2 expression has a strong association with higher fertilization rate. Further studies are needed to support this theory.
URI: http://dx.doi.org/10.1186/s12958-015-0099-8
http://hdl.handle.net/11536/128135
ISSN: 1477-7827
DOI: 10.1186/s12958-015-0099-8
期刊: REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
Volume: 13
起始頁: 0
結束頁: 0
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