標題: An aberrant autocrine activation of the platelet-derived growth factor alpha-receptor in follicular and papillary thyroid carcinoma cell lines
作者: Chen, KT
Lin, JD
Liou, MJ
Weng, HF
Chang, CA
Chan, EC
生物科技學系
Department of Biological Science and Technology
關鍵字: platelet-derived growth factor-A;platelet-derived growth factor alpha-receptor;autocrine activation;thyroid carcinoma cell line
公開日期: 18-Jan-2006
摘要: Platelet-derived growth factor receptor (PDGFR) can bind to its ligand and consequently possess a kinase activity, and which is associated with the carcinogenesis of different cell types, including astrocytomas, oligodendrogliomas. and glioblastoma. In a cDNA microarray analysis, we observe the over-expressed mRNA of both PDGF-A and PDGF-alpha receptor in thyroid carcinoma cells. And the elevated protein expressions of PDGF-A and PDGF-alpha receptor in thyroid carcinoma cells were also confirmed by a Western blot analysis. The phosphorylation of PDGF-alpha receptor evaluated by an antibody against Tyr 720-phosphate was found in thyroid carcinoma cells. The tyrosine kinase activity of PDGF-alpha receptor was inhibited by tyrphostin AG1295 and showed a dose-dependent inhibition for the proliferation of thyroid carcinoma cells. These findings imply that autocrine activation of PDGF-alpha receptor plays a crucial role in the carcinogenesis of thyroid cells. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.canlet.2005.01.039
http://hdl.handle.net/11536/12728
ISSN: 0304-3835
DOI: 10.1016/j.canlet.2005.01.039
期刊: CANCER LETTERS
Volume: 231
Issue: 2
起始頁: 192
結束頁: 205
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