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dc.contributor.authorHung, YCen_US
dc.contributor.authorHong, MYen_US
dc.contributor.authorHuang, GSen_US
dc.date.accessioned2014-12-08T15:17:24Z-
dc.date.available2014-12-08T15:17:24Z-
dc.date.issued2006-02-06en_US
dc.identifier.issn0014-5793en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.febslet.2005.12.102en_US
dc.identifier.urihttp://hdl.handle.net/11536/12637-
dc.description.abstractTo investigate the molecular consequence of loading free cholesterol into macrophages, we conducted a large-scale gene expression study to analyze acetylated-LDL-laden foam cells (AFC) and oxidized-LDL-laden foam cells (OFC) induced from human THP-1 cell lines. Cluster analysis was performed using 9600-gene microarray datasets from time course experiment. AFC and OFC shared common expression profiles; however, there were sufficient differences between these two treatments that AFC and OFC appealed as two separate entities. We identified 80 commonly upregulated genes and 48 commonly downregulated genes in AFC and OFC. Functional annotation of the differentially expressed genes indicated that apoptosis, extracellular matrix, oxidative stress, and cell proliferation was deregulated. We also identified 87 differentially expressed genes unique for AFC and 31 genes for OFC. The uniquely expressed genes of AFC are associated with kinase activity, ATP binding activity, and transporter activity, while unique genes for OFC are associated with cell signaling and adhesion. To validate the hypothesis that oxidative stress is a common feature for AFC and OFC, we performed a cluster analysis employing the genes related to oxidative stress, but we were unable to distinguish AFC from OFC in this manner. We performed real-time RTPCR and ELISA on foam cells to examine the transcripts and secreted protein of interleukin 1 beta (IL1 beta). IL1 beta was rapidly induced in foam cells, but for AFC both RNA level and protein level dropped immediately and was attenuated. To detect levels of reactive oxygen species in foam cells we conducted hydroethidine staining and observed high levels of superoxide anion. We conclude that loading free cholesterol induces high levels of superoxide anion, increases oxidative stress, and triggers a transient inflammatory response in macrophages. (c) 2006 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.en_US
dc.language.isoen_USen_US
dc.subjectlow-density lipoproteinsen_US
dc.subjectfoam cellsen_US
dc.subjectmicroarrayen_US
dc.subjectatherosclerosisen_US
dc.subjectmacrophagesen_US
dc.subjectoxidized LDLen_US
dc.subjectacetylated LDLen_US
dc.subjectapoptosisen_US
dc.subjectexpression patternen_US
dc.subjectinflammatory responseen_US
dc.subjectreal-time RT-PCRen_US
dc.titleCholesterol loading augments oxidative stress in macrophagesen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.febslet.2005.12.102en_US
dc.identifier.journalFEBS LETTERSen_US
dc.citation.volume580en_US
dc.citation.issue3en_US
dc.citation.spage849en_US
dc.citation.epage861en_US
dc.contributor.department材料科學與工程學系奈米科技碩博班zh_TW
dc.contributor.departmentGraduate Program of Nanotechnology , Department of Materials Science and Engineeringen_US
dc.identifier.wosnumberWOS:000235222500021-
dc.citation.woscount15-
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