標題: A turn-on fluorescent pyrene-based chemosensor for Cu(II) with live cell application
作者: Venkatesan, Parthiban
Wu, Shu-Pao
應用化學系
Department of Applied Chemistry
公開日期: 1-一月-2015
摘要: A pyrene-based fluorescent sensor (PHP) was synthesized for Cu(II) detection. It had high selectivity towards Cu2+ ions via photoinduced electron transfer (PET) based fluorescence enhancement. In the presence of Cu2+, PHP provided significant blue emission, while Ag+, Al3+, Ca2+, Cd2+, Co2+, Cr3+, Fe2+, Fe3+, Hg2+, Mg2+, Mn2+, Ni2+, Pb2+, and Zn2+ metal ions produced only minor changes in the fluorescence spectra. The association constant (K-a) for Cu2+ binding to PHP had a value of 1.0 x 10(4) M-1. The maximum emission change induced by Cu2+ binding to the chemosensor PHP was observed over the pH range 5.0-10.0. Confocal fluorescence microscopy imaging using RAW264.7 cells showed that PHP can be used as an effective fluorescent probe for detecting Cu2+ in living cells.
URI: http://dx.doi.org/10.1039/c5ra05440k
http://hdl.handle.net/11536/124865
ISSN: 2046-2069
DOI: 10.1039/c5ra05440k
期刊: RSC ADVANCES
Issue: 53
起始頁: 42591
結束頁: 42596
顯示於類別:期刊論文