標題: 3D Porous Calcium-Alginate Scaffolds Cell Culture System Improved Human Osteoblast Cell Clusters for Cell Therapy
作者: Chen, Ching-Yun
Ke, Cherng-Jyh
Yen, Ko-Chung
Hsieh, Hui-Chen
Sun, Jui-Sheng
Lin, Feng-Huei
生醫工程研究所
智慧型仿生系統研究中心
Institute of Biomedical Engineering
Biomimetic Systems Research Center
關鍵字: Three-dimensional culture;Preformed scaffolds;Bone-like tissues;Perfusion;Auto-grafts
公開日期: 1-Jan-2015
摘要: Age-related orthopedic disorders and bone defects have become a critical public health issue, and cell-based therapy is potentially a novel solution for issues surrounding bone tissue engineering and regenerative medicine. Long-term cultures of primary bone cells exhibit phenotypic and functional degeneration; therefore, culturing cells or tissues suitable for clinical use remain a challenge. A platform consisting of human osteoblasts (hOBs), calcium-alginate (Ca-Alginate) scaffolds, and a self-made bioreactor system was established for autologous transplantation of human osteoblast cell clusters. The Ca-Alginate scaffold facilitated the growth and differentiation of human bone cell clusters, and the functionally-closed process bioreactor system supplied the soluble nutrients and osteogenic signals required to maintain the cell viability. This system preserved the proliferative ability of cells and cell viability and up-regulated bone-related gene expression and biological apatite crystals formation. The bone-like tissue generated could be extracted by removal of calcium ions via ethylenediaminetetraacetic acid (EDTA) chelation, and exhibited a size suitable for injection. The described strategy could be used in therapeutic application and opens new avenues for surgical interventions to correct skeletal defects.
URI: http://dx.doi.org/10.7150/thno.11372
http://hdl.handle.net/11536/124739
ISSN: 1838-7640
DOI: 10.7150/thno.11372
期刊: THERANOSTICS
Volume: 5
Issue: 6
起始頁: 643
結束頁: 655
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