標題: Tryptophan 232 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences rearrangement and deprotonation but not cyclization reactions
作者: Wu, TK
Yu, MT
Liu, YT
Chang, CH
Wang, HJ
Diau, EWG
生物科技學系
應用化學系
Department of Biological Science and Technology
Department of Applied Chemistry
公開日期: 30-Mar-2006
摘要: Oxidosqualene-lanosterol cyclases convert oxidosqualene to lanosterol in yeast and mammals. Site-saturated mutants' construction of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase, at Trp232 exchanges against proteinogenic amino acids, and product profiles are shown. All mutants, except Lys and Arg, produced protosta-12,24-dien-3 beta-ol, lanosterol, and parkeol. Overall, Trp232 plays a catalytic role in the influence of rearrangement process and determination of deprotonation position but does not involve intervention in the cyclization steps.
URI: http://dx.doi.org/10.1021/ol053134w
http://hdl.handle.net/11536/12470
ISSN: 1523-7060
DOI: 10.1021/ol053134w
期刊: ORGANIC LETTERS
Volume: 8
Issue: 7
起始頁: 1319
結束頁: 1322
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