標題: Sweeping technique combined with micellar electrokinetic chromatography for the simultaneous determination of flunitrazepam and its major metabolites
作者: Huang, CW
Jen, HP
Wang, RD
Hsieh, YZ
應用化學系
Department of Applied Chemistry
關鍵字: sweeping;micellar electrokinetic chromatography;flunitrazepam;cetyltrimethylammonium bromide
公開日期: 31-Mar-2006
摘要: A sweeping technique, in conjunction with micellar electrokinetic chromatography, for the simultaneous determination of flunitrazepam and its major metabolites, 7-aminoflunitrazepam and N-desmethylflunitrazepam, is described. The optimized conditions for the sweeping and separation were a pH 9.5 buffer, 25 mM borate, 50 mM cetyltrimethylammonium bromide, 30% MeOH (v/v), and a 151-mm injection length. The calibration functions were all linear with the coefficient of determination (r(2)) exceeding 0.996 for the three target compounds. Using the sweeping procedure, the limits of detection were determined to be 13.4, 5.6, and 12.0 ng/mL for flunitrazepam, 7-aminoflunitrazepam, and N-desmethylflunitrazepam, respectively, and the sensitivity enhancement for each compound was within the range of 110-200 fold. The RSDs for the retention time and the peak area were less than 4.10%. The optimized sweeping method was also used to examine a spiked urine sample. We conclude that sweeping with micellar electrokinetic chromatography has considerable potential use in clinical and forensic analyses of flunitrazepam and its metabolites. (c) 2006 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.chroma.2006.01.070
http://hdl.handle.net/11536/12469
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2006.01.070
期刊: JOURNAL OF CHROMATOGRAPHY A
Volume: 1110
Issue: 1-2
起始頁: 240
結束頁: 244
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