Title: Biological imaging with nonlinear photothermal microscopy using a compact supercontinuum fiber laser source
Authors: He, Jinping
Miyazaki, Jun
Wang, Nan
Tsurui, Hiromichi
Kobayashi, Takayoshi
Department of Electrophysics
Issue Date: 20-Apr-2015
Abstract: Nonlinear photothermal microscopy is applied in the imaging of biological tissues stained with chlorophyll and hematoxylin. Experimental results show that this type of organic molecules, which absorb light but transform dominant part of the absorbed energy into heat, may be ideal probes for photothermal imaging without photochemical toxicity. Picosecond pump and probe pulses, with central wavelengths of 488 and 632 nm, respectively, are spectrally filtered from a compact supercontinuum fiber laser source. Based on the light source, a compact and sensitive super-resolution imaging system is constructed. Further more, the imaging system is much less affected by thermal blurring than photothermal microscopes with continuous-wave light sources. The spatial resolution of nonlinear photothermal microscopy is similar to 188 nm. It is similar to 23% higher than commonly utilized linear photothermal microscopy experimentally and similar to 43% than conventional optical microscopy theoretically. The nonlinear photothermal imaging technology can be used in the evaluation of biological tissues with high-resolution and contrast. (C) 2015 Optical Society of America
URI: http://dx.doi.org/10.1364/OE.23.009762
ISSN: 1094-4087
DOI: 10.1364/OE.23.009762
Volume: 23
Begin Page: 9762
End Page: 9771
Appears in Collections:Articles