Title: Sub-diffraction-limit imaging using mode multiplexing
Authors: Wang, Nan
Miyazaki, Jun
He, Jinping
Seto, Keisuke
Kobayashi, Takayoshi
Department of Electrophysics
Keywords: Confocal microscopy;Mode multiplexing;Superresolution;Image processing
Issue Date: 15-May-2015
Abstract: Pixel-by-pixel processed fluorescence difference microscopy is experimentally demonstrated by multiplexing excitation laser beams with Gaussian and donut spot shapes and then demultiplexing the fluorescent signals using lock-in amplifiers. With this scheme, a fixed sample of fluorescent spheres and a slice of mouse brain tissue are imaged with resolutions that exceed the diffraction limit. Compared to previously reported subtraction imaging techniques, this pixel-by-pixel scan can be applied to improve the resolution of a moving sample without introducing subtraction errors. The synchronized signal detection feature makes this method extendible to various applications. (C) 2014 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.optcom.2014.12.072
ISSN: 0030-4018
DOI: 10.1016/j.optcom.2014.12.072
Volume: 343
Begin Page: 28
End Page: 33
Appears in Collections:Articles