Title: A fluorescent turn-on probe for Hg(II) based on an NTe2 chelating motif and its application in living cell imaging
Authors: Kao, Shao-Lun
Wu, Shu-Pao
Department of Applied Chemistry
Keywords: Sensors;Hg(II);Fluorescence;Imaging agents
Issue Date: 1-Jun-2015
Abstract: A boron dipyrromethene (BODIPY) derivative (BODTe) with an NTe2 chelating motif exhibits high selectivity for Hg2+ detection. Significant fluorescence enhancement was observed with the chemosensor BODTe in the presence of Hg2+. However, the metal ions Ag+, Al3+, Ca2+, Cd2+, Co2+, Cu2+,Cr3+, Fe2+, Fe3+, Hg2+, Mg2+, Mn2+, Ni2+, Pb2+, and Zn2+ produced only minor changes in the fluorescence values of the system. The association constant (Ka) of Hg2+ binding for the chemosensor BODTe was found to be 3.84 x 10(3) M-1, with a detection limit of 19.2 mu M. The maximum fluorescence enhancement caused by Hg2+ binding in the chemosensor BODTe was observed over the pH range of 4.0-10.0. Confocal fluorescence microscopy imaging using RAW 264.7 cells showed that BODTe can be used as an effective fluorescent probe for detecting Hg2+ in living cells. (C) 2015 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.snb.2015.02.053
ISSN: 0925-4005
DOI: 10.1016/j.snb.2015.02.053
Volume: 212
Begin Page: 382
End Page: 388
Appears in Collections:Articles