Title: Simultaneous dual-wavelength imaging of nonfluorescent tissues with 3D subdiffraction photothermal microscopy
Authors: Miyazaki, Jun
Tsurui, Hiromichi
Kawasumi, Koshi
Kobayashi, Takayoshi
Department of Electrophysics
Issue Date: 9-Feb-2015
Abstract: Multi-wavelength microscopic imaging is essential to visualize a variety of nanoscale cellular components with high specificity and high spatial resolution. However, previous techniques are based on fluorescence, and thus cannot visualize nonfluorescent species, which are much less suffered from photodamage or photobleaching and hence are intrinsically useful in wider range of optical microscopy. Here, we show that simultaneous multi-wavelength imaging of nonfluorescent species can be achieved with the use of a photothermal microscope. Dual-wavelength subdiffraction imaging of biological tissues stained with hematoxylin and eosin is demonstrated. Three-dimensional label-free imaging of mouse melanoma tissue section is also presented to demonstrate the effectiveness of the enhanced spatial resolution. Our technique can be implemented using cost-effective and compact laser diodes and is applicable for various types of both fluorescent and nonfluorescent tissues. (C) 2015 Optical Society of America
URI: http://dx.doi.org/10.1364/OE.23.003647
ISSN: 1094-4087
DOI: 10.1364/OE.23.003647
Volume: 23
Begin Page: 3647
End Page: 3656
Appears in Collections:Articles