標題: Simultaneous dual-wavelength imaging of nonfluorescent tissues with 3D subdiffraction photothermal microscopy
作者: Miyazaki, Jun
Tsurui, Hiromichi
Kawasumi, Koshi
Kobayashi, Takayoshi
電子物理學系
Department of Electrophysics
公開日期: 9-二月-2015
摘要: Multi-wavelength microscopic imaging is essential to visualize a variety of nanoscale cellular components with high specificity and high spatial resolution. However, previous techniques are based on fluorescence, and thus cannot visualize nonfluorescent species, which are much less suffered from photodamage or photobleaching and hence are intrinsically useful in wider range of optical microscopy. Here, we show that simultaneous multi-wavelength imaging of nonfluorescent species can be achieved with the use of a photothermal microscope. Dual-wavelength subdiffraction imaging of biological tissues stained with hematoxylin and eosin is demonstrated. Three-dimensional label-free imaging of mouse melanoma tissue section is also presented to demonstrate the effectiveness of the enhanced spatial resolution. Our technique can be implemented using cost-effective and compact laser diodes and is applicable for various types of both fluorescent and nonfluorescent tissues. (C) 2015 Optical Society of America
URI: http://dx.doi.org/10.1364/OE.23.003647
http://hdl.handle.net/11536/124331
ISSN: 1094-4087
DOI: 10.1364/OE.23.003647
期刊: OPTICS EXPRESS
Volume: 23
起始頁: 3647
結束頁: 3656
顯示於類別:期刊論文