標題: Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development
作者: Hu, Jie-Bi
Chen, Ting-Ru
Chen, Yu-Chie
Urban, Pawel L.
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 30-Jan-2015
摘要: In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2-48 mu M. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.
URI: http://dx.doi.org/10.1038/srep08135
http://hdl.handle.net/11536/124201
ISSN: 2045-2322
DOI: 10.1038/srep08135
期刊: SCIENTIFIC REPORTS
Volume: 5
起始頁: 0
結束頁: 0
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