Title: Optimal detection angle in sub-diffraction resolution photothermal microscopy: application for high sensitivity imaging of biological tissues
Authors: Miyazaki, Jun
Tsurui, Hiromichi
Kawasumi, Koshi
Kobayashi, Takayoshi
Department of Electrophysics
Issue Date: 11-Aug-2014
Abstract: We evaluated the optimal detection angle for maximizing the signal to noise ratio (SNR) in sub-diffraction resolution photothermal microscopy. The angular dependent photothermal signal was calculated based on scattering theory using the temporally modulated Yukawa potential, and its detection angle and modulation frequency dependencies were analyzed. We verified the theoretical findings by imaging gold nanoparticles using laser diode based photothermal microscopy with balanced detection scheme. High-sensitivity (SNR similar to 40) photothermal biological imaging of a mouse brain was also demonstrated.
URI: http://dx.doi.org/10.1364/OE.22.018833
ISSN: 1094-4087
DOI: 10.1364/OE.22.018833
Volume: 22
Issue: 16
Appears in Collections:Articles