標題: Metabolic variation of HeLa cells migrating on microfabricated cytophilic channels studied by the fluorescence lifetime of NADH
作者: Deka, Gitanjal
Okano, Kazunori
Masuhara, Hiroshi
Li, Yaw-Kuen
Kao, Fu-Jen
應用化學系
應用化學系分子科學碩博班
光電工程學系
Department of Applied Chemistry
Institute of Molecular science
Department of Photonics
公開日期: 1-一月-2014
摘要: We report a novel method for studying cellular migration in vitro. Cytophilic microdomains were formed on a cytophobic substrate by laser ablation. HeLa cells were grown on those domains until confluence, and then channels were formed to guide cellular migration. Two-photon excitation fluorescence-lifetime imaging of NADH revealed metabolic variation among migrating and nonmigrating cells.
URI: http://dx.doi.org/10.1039/c4ra06492e
http://hdl.handle.net/11536/124003
ISSN: 2046-2069
DOI: 10.1039/c4ra06492e
期刊: RSC ADVANCES
Issue: 83
起始頁: 44100
結束頁: 44104
顯示於類別:期刊論文


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