標題: The effect of fluorescent nanodiamonds on neuronal survival and morphogenesis
作者: Huang, Yung-An
Kao, Chun-Wei
Liu, Kuang-Kai
Huang, Hou-Syun
Chiang, Ming-Han
Soo, Ching-Ren
Chang, Huan-Cheng
Chiu, Tzai-Wen
Chao, Jui-I
Hwang, Eric
生物科技學系
生物資訊及系統生物研究所
分子醫學與生物工程研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
Institute of Molecular Medicine and Bioengineering
公開日期: 5-十一月-2014
摘要: Nanodiamond (ND) has emerged as a promising carbon nanomaterial for therapeutic applications. In previous studies, ND has been reported to have outstanding biocompatibility and high uptake rate in various cell types. ND containing nitrogen-vacancy centers exhibit fluorescence property is called fluorescent nanodiamond (FND), and has been applied for bio-labeling agent. However, the influence and application of FND on the nervous system remain elusive. In order to study the compatibility of FND on the nervous system, neurons treated with FNDs in vitro and in vivo were examined. FND did not induce cytotoxicity in primary neurons from either central (CNS) or peripheral nervous system (PNS); neither did intracranial injection of FND affect animal behavior. The neuronal uptake of FNDs was confirmed using flow cytometry and confocal microscopy. However, FND caused a concentration-dependent decrease in neurite length in both CNS and PNS neurons. Time-lapse live cell imaging showed that the reduction of neurite length was due to the spatial hindrance of FND on advancing axonal growth cone. These findings demonstrate that FNDs exhibit low neuronal toxicity but interfere with neuronal morphogenesis, and should be taken into consideration when applications involve actively growing neurites (e.g. nerve regeneration).
URI: http://dx.doi.org/10.1038/srep06919
http://hdl.handle.net/11536/123917
ISSN: 2045-2322
DOI: 10.1038/srep06919
期刊: SCIENTIFIC REPORTS
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