標題: Crowning Proteins: Modulating the Protein Surface Properties using Crown Ethers
作者: Lee, Cheng-Chung
Maestre-Reyna, Manuel
Hsu, Kai-Cheng
Wang, Hao-Ching
Liu, Chia-I
Jeng, Wen-Yih
Lin, Li-Ling
Wood, Richard
Chou, Chia-Cheng
Yang, Jinn-Moon
Wang, Andrew H. -J.
生物資訊及系統生物研究所
Institude of Bioinformatics and Systems Biology
關鍵字: crown compounds;crystal growth;molecular dynamics;protein engineering;protein surfaces
公開日期: 24-十一月-2014
摘要: Crown ethers are small, cyclic polyethers that have found wide-spread use in phase-transfer catalysis and, to a certain degree, in protein chemistry. Crown ethers readily bind metallic and organic cations, including positively charged amino acid side chains. We elucidated the crystal structures of several protein-crown ether co-crystals grown in the presence of 18-crown-6. We then employed biophysical methods and molecular dynamics simulations to compare these complexes with the corresponding apoproteins and with similar complexes with ring-shaped low-molecular-weight polyethylene glycols. Our studies show that crown ethers can modify protein surface behavior dramatically by stabilizing either intra- or intermolecular interactions. Consequently, we propose that crown ethers can be used to modulate a wide variety of protein surface behaviors, such as oligomerization, domain-domain interactions, stabilization in organic solvents, and crystallization.
URI: http://dx.doi.org/10.1002/anie.201405664
http://hdl.handle.net/11536/123894
ISSN: 1433-7851
DOI: 10.1002/anie.201405664
期刊: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume: 53
Issue: 48
起始頁: 13054
結束頁: 13058
顯示於類別:期刊論文


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