標題: Heavy chain of cytoplasmic dynein is a major component of the postsynaptic density fraction
作者: Cheng, Huei-Hsuan
Liu, Szu-Heng
Lee, Flui-Chen
Lin, Ya-Shiuan
Huang, Zu-Han
Hsu, Cheng-I
Chen, Yu-Chie
Chang, Yen-Chung
應用化學系
Department of Applied Chemistry
關鍵字: MALDI-TOF;subcellular fractions;dendritic spines
公開日期: 1-八月-2006
摘要: A protein with an apparent molecular size of 490 kDa was found in the postsynaptic density (PSD) fraction isolated from porcine cerebral cortices and rat forebrains, and this 490 kDa protein accounted for similar to 3% of the total protein of these samples. Matrix-assisted laser desorption ionization-time of flight mass spectrometric and Western blotting analyses consistently indicated that this 490 kDa protein consisted primarily of the heavy chain of cytoplasmic dynein (cDHC). Immunocytochemical analyses showed that cDHC was found in 92% and 89% of the phalloidin-positive protrusions that were themselves associated with discrete clusters of synaptophysin, a presynaptic terminal marker, and PSD-95, a postsynaptic marker, on neuronal processes, respectively. Quantitative Western blotting analyses of various subcellular fractions isolated from porcine cerebral cortices and rat forebrains further showed that not only the heavy but also the intermediate chains of dynein are enriched in the PSD fraction. Cytoplasmic dynein is a microtubule-associated motor protein complex that drives the movement of various cargos toward the minus ends of microtubules and plays many other diverse functions in the cell. Our results that cDHC is a major component of the PSD fraction, that both dynein heavy and intermediate chains are enriched in the PSD fraction and that cDHC is present in dendritic spines raise the possibilities that cytoplasmic dynein may play structural and functional roles in the postsynaptic terminal. (c) 2006 Wiley-Liss, Inc.
URI: http://dx.doi.org/10.1002/jnr.20898
http://hdl.handle.net/11536/11931
ISSN: 0360-4012
DOI: 10.1002/jnr.20898
期刊: JOURNAL OF NEUROSCIENCE RESEARCH
Volume: 84
Issue: 2
起始頁: 244
結束頁: 254
顯示於類別:期刊論文


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