標題: Counter-current chromatography using hexane/surfactant-containing water solvent systems
作者: Shen, Ching-Wei
Boon, Siew-Keem
Chang, Yu-Pei
Yu, Tiing
應用化學系
Department of Applied Chemistry
關鍵字: counter-current chromatography;solvent systems;surfactant;micelle
公開日期: 4-Aug-2006
摘要: We developed a n-hexane/surfactant-containing water solvent system in counter-current chromatography (CCC) in order to separate hydrophobic compounds. By using the upper phase as the mobile phase, we have separated steroid samples. Retention times of steroids progesterone and Delta(4)-androstene-3,17-dione increased slightly by increasing the concentration below the critical micellar concentration (CMC) of surfactant sodium 1-heptanesulfonate. However, the retention times increased drastically while the SHS concentrations were above the CMC. The partition of these two steroids in the two phases was significantly dependent on the interaction with micelles. Aromatic hydrocarbons were not retained by the lower phase no matter what the surfactant concentrations were. Their hydrophobic interaction with n-hexane greatly exceeded that with the micellar solution. The retention times of esters, however, were only slightly affected by the surfactant addition even above the CMC. The weaker interaction between esters and the micellar solution was probably due to their higher polarity. The micellar solvent systems provide an alternative way for hydrophobic sample separations in CCC, but the performance is limited. (c) 2006 Published by Elsevier B.V.
URI: http://dx.doi.org/10.1016/j.chroma.2006.05.007
http://hdl.handle.net/11536/11929
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2006.05.007
期刊: JOURNAL OF CHROMATOGRAPHY A
Volume: 1123
Issue: 1
起始頁: 66
結束頁: 70
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