標題: Purification, crystallization and preliminary X-ray crystallographic analysis of chitinase from Bacillus cereus NCTU2
作者: Kuo, Chueh-Yuan
Wu, Yue-Jin
Hsieh, Yin-Cheng
Guan, Hong-Hsiang
Tsai, Huei-Ju
Lin, Yi-Hung
Huang, Yen-Chieh
Liu, Ming-Yih
Li, Yaw-Kuen
Chen, Chun-Jung
應用化學系
Department of Applied Chemistry
公開日期: 1-Sep-2006
摘要: Chitinases (EC 3.2.1.14) are found in a broad range of organisms, including bacteria, fungi and higher plants, and play different roles depending on their origin. A chitinase from Bacillus cereus NCTU2 (ChiNCTU2) capable of hydrolyzing chitin as a carbon and nitrogen nutrient has been identified as a member of the family 18 glycoside hydrolases. ChiNCTU2 of molecular weight 36 kDa has been crystallized using the hanging-drop vapour-diffusion method. According to the diffraction of chitinase crystals at 1.10 angstrom resolution, the crystal belongs to space group P2(1), with unit-cell parameters a = 50.79, b = 48.79, c = 66.87 angstrom, beta = 99.31 degrees. Preliminary analysis indicates there is one chitinase molecule in the asymmetric unit, with a solvent content of 43.4%.
URI: http://dx.doi.org/10.1107/S1744309106031423
http://hdl.handle.net/11536/11807
ISSN: 1744-3091
DOI: 10.1107/S1744309106031423
期刊: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
Volume: 62
Issue: 
起始頁: 916
結束頁: 919
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