標題: Phenylalanine 445 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences C-ring cyclization and deprotonation reactions
作者: Wu, Tung-Kung
Liu, Yuan-Ting
Chiu, Feng-Hsuan
Chang, Cheng-Hsiang
生物科技學系
Department of Biological Science and Technology
公開日期: 12-Oct-2006
摘要: We describe the Saccharomyces cerevisiae oxidosqualene - lanosterol cyclase Phe445 site-saturated mutants that generate truncated tricyclic and altered deprotonation product profiles. Among these mutants, only polar side-chain group substitutions genetically complemented yeast viability and produced spatially related product diversity, supporting the Johnson model that cation - pi interactions between a carbocationic intermediate and an enzyme can be replaced by an electrostatic or polar side chain to stabilize the cationic intermediate, but with product differentiation.
URI: http://dx.doi.org/10.1021/ol061549r
http://hdl.handle.net/11536/11677
ISSN: 1523-7060
DOI: 10.1021/ol061549r
期刊: ORGANIC LETTERS
Volume: 8
Issue: 21
起始頁: 4691
結束頁: 4694
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