標題: Regulation of the homologous two-component systems KvgAS and KvhAS in Klebsiella pneumoniae CG43
作者: Lin, Ching-Ting
Peng, Hwei-Ling
生物科技學系
Department of Biological Science and Technology
關鍵字: fosfomycin;katG;KvgAS;KvhAS;rpoS;sodC;two-component system
公開日期: 1-Nov-2006
摘要: In Klebsiella pneumoniae CG43, deletion of the sensor gene kvgS reduced the kvgAS expression in M9 medium with 0.2 mM paraquat, 0.2 mM 2,2-dihydropyridyl, or 300 mM NaCl. This result shows an autoregulatory role of KvgS and a stress-responsive expression of the two-component system (2CS). The kvgS deletion also appeared to decrease the expression of kvhAS, paralogous genes of kvgAS. Additionally, measurements of the promoter activity in kvgA(-) mutant revealed that KvgA is probably an activator for the expression of kvgAS and kvhAS. The subsequent electrophoretic mobility shift assay, indicating a specific binding of the recombinant KvgA to the putative promoters P-kvgAS and P-kvhAs, also supported an interacting regulation between the 2CSs. In P-kvgAs and P-kvhAS, the presence of RpoS binding elements suggested an RpoS-dependent regulation. Nevertheless, the rpoS deletion reduced the expression of kvgAS but increased that of kvhAS. Moreover, the kvgA deletion reduced the expression of katG and sodC. The overexpression of KvhA altered the susceptibility to fosfomycin and an increasing activity of UDP-N-acetylglucosamine enolpyruvyl transferase, the target protein of fosfomycin, which suggesting a regulation by KvhA. Taken together, these indicated that the two 2CSs probably belong to different regulatory circuits of the RpoS regulon.
URI: http://dx.doi.org/10.1093/jb/mvj196
http://hdl.handle.net/11536/11620
ISSN: 0021-924X
DOI: 10.1093/jb/mvj196
期刊: JOURNAL OF BIOCHEMISTRY
Volume: 140
Issue: 5
起始頁: 639
結束頁: 648
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