Title: Measuring the flexibility of immunoglobulin by gold nanoparticles
Authors: Huang, G. Steven
Chen, Yu-Shiun
Yeh, Hsiao-Wei
Graduate Program of Nanotechnology , Department of Materials Science and Engineering
Center for Nanoscience and Technology
Issue Date: 8-Nov-2006
Abstract: We measured the flexibility of Fab and Fc arms of immunoglobulin using gold nanoparticles (GNPs). Enzyme-linked immunosorbent assay was performed to measure the affinity of anti-5 nm GNP antiserum against various sizes of GNPs. The flexibility of Fc was also measured by electron microscopy. The restricted binding affinity indicated that only a very limited amount of freedom was allowed for the Fab-Fab hinge, while Fab-Fc showed a much larger degree of freedom.
URI: http://dx.doi.org/10.1021/nl061598x
ISSN: 1530-6984
DOI: 10.1021/nl061598x
Volume: 6
Issue: 11
Begin Page: 2467
End Page: 2471
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