標題: Measuring the flexibility of immunoglobulin by gold nanoparticles
作者: Huang, G. Steven
Chen, Yu-Shiun
Yeh, Hsiao-Wei
材料科學與工程學系奈米科技碩博班
奈米科技中心
Graduate Program of Nanotechnology , Department of Materials Science and Engineering
Center for Nanoscience and Technology
公開日期: 8-十一月-2006
摘要: We measured the flexibility of Fab and Fc arms of immunoglobulin using gold nanoparticles (GNPs). Enzyme-linked immunosorbent assay was performed to measure the affinity of anti-5 nm GNP antiserum against various sizes of GNPs. The flexibility of Fc was also measured by electron microscopy. The restricted binding affinity indicated that only a very limited amount of freedom was allowed for the Fab-Fab hinge, while Fab-Fc showed a much larger degree of freedom.
URI: http://dx.doi.org/10.1021/nl061598x
http://hdl.handle.net/11536/11555
ISSN: 1530-6984
DOI: 10.1021/nl061598x
期刊: NANO LETTERS
Volume: 6
Issue: 11
起始頁: 2467
結束頁: 2471
顯示於類別:期刊論文


文件中的檔案:

  1. 000241856700014.pdf