Title: Increased expression of nuclear NF-kappa B after coronary artery balloon injury can be inhibited by intracoronary beta-irradiation
Authors: Su, Chun-Hui
Hsieh, Bor-Tsung
Leung, Stephen-Wan
Wu, Chiung-Jen
Chiu, Sheng-Yi
Lin, Chih-Sheng
Department of Biological Science and Technology
Keywords: NF-kappa B;coronary artery;restenosis;rhenium-188;intracoronary brachytherapy
Issue Date: 2007
Abstract: Purpose: Molecular mechanisms by which balloon angioplasty injury-induced neointimal hyperplasia can be reduced by intravascular brachytherapy are unclear. We investigated the role of nuclear factor-kappaB (NF-B-K) in neointimal hyperplasia following intracoronary irradiation. Materials and methods: Fifty-four coronary arteries from 30 pigs were divided into 6 groups: sham control, balloon angioplasty injury alone, beta-irradiation at doses of 14 or 20 Gy, and 14 or 20 Gy beta-irradiation immediately followed by balloon injury. Coronary arteries were injured by overstretch balloon angioplasty and then the arteries were irradiated using a Rhenium-188 (188 Re) b-emitting solution-filled balloon. Pigs were scarified one day or one week after coronary interventions for molecular detection and six weeks after the procedures for histological examination. Results: Six weeks after coronary interventions, the histological results show that balloon angioplasty injury had induced intimal hyperplasia in coronary artery but the response was significantly reduced by 28% and 60% when the injury was immediately treated by 14 and 20 Gy 188 Re beta-irradiation, respectively. The expression of arterial NF-B-K p65, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) were detected at one day and one week after the procedures. The treatment of balloon injury could significantly induce the NF-kB p65 expression in both gene and protein levels, and such induction could be significantly reduced by 188 Re beta-irradiation at dose of 20 Gy. However, the similar result on the regulation of gene expression affected by the b- irradiation could not be observed in ICAM-1 and VCAM-1. Conclusion: The inhibitory effect of intracoronary brachytherapy on neointimal formation following overstretch balloon angioplasty could involve inhibition of NF-kB p65.
URI: http://hdl.handle.net/11536/11252
ISSN: 0955-3002
DOI: 10.1080/09553000701596126
Volume: 83
Issue: 10
Begin Page: 707
End Page: 716
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